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Old 04-13-2015, 07:59 AM   #1
Spleen
Junior Member
 
Location: germany

Join Date: Feb 2010
Posts: 1
Question Bioanalyzer, empty wells show profiles.

Dear All.
It has occurred in the last couple of run that empty wells in the bioanalyzer actually showed a RNA profile (indistinguishable from a real sample)
Here some details:
-RNA pico chip
-It occurred 3 times (weeks apart) every time in a different well.
-I run a test with only empty wells and one positive control in the central well, there was no contamination.
-The "ghost samples" in one case was very similar to a real sample even though these were not adjoin (well 6 and 10 ).

Has anyone experienced anything like that before that could not be due to a mis-pipetting ( even though I can imagine a mis-pipetting I really don't think so) ?

Thank you very much and have a good day!
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Old 04-13-2015, 10:43 AM   #2
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,236
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Quote:
Originally Posted by Spleen View Post
Dear All.
It has occurred in the last couple of run that empty wells in the bioanalyzer actually showed a RNA profile (indistinguishable from a real sample)
Here some details:
-RNA pico chip
-It occurred 3 times (weeks apart) every time in a different well.
-I run a test with only empty wells and one positive control in the central well, there was no contamination.
-The "ghost samples" in one case was very similar to a real sample even though these were not adjoin (well 6 and 10 ).

Has anyone experienced anything like that before that could not be due to a mis-pipetting ( even though I can imagine a mis-pipetting I really don't think so) ?

Thank you very much and have a good day!
If you used the same electrode for a nano-RNA chip prior to running a pico-RNA chip then the pins would need to be very carefully washed. The amount of RNA needed to visualize on a pico-RNA chip is very small.

--
Phillip

Last edited by pmiguel; 04-13-2015 at 10:47 AM.
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Old 03-04-2016, 11:14 AM   #3
sajoshi
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Location: cleveland

Join Date: Dec 2015
Posts: 26
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Clean the pins with a brush. Alternate with propanol and milliq water a few times and try again.
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