SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
PhiX removal jamimo Illumina/Solexa 5 04-06-2015 05:50 AM
less PhiX than you think? beardy_man Illumina/Solexa 1 11-08-2013 12:50 PM
PhiX contamination MoritzF Illumina/Solexa 3 08-06-2013 05:49 PM
V3 PhiX kthai Illumina/Solexa 0 04-01-2011 12:21 PM
PhiX Spiking DavidBaker Illumina/Solexa 0 09-24-2010 08:35 AM

Reply
 
Thread Tools
Old 08-25-2015, 11:49 AM   #1
jwfoley
Senior Member
 
Location: Stanford

Join Date: Jun 2009
Posts: 176
Post phiX on Bioanalyzer

In case anyone else was curious. This is Illumina's ΦX174 reference library ("PhiX Sequencing Control V3", p/n FC-110-3001, allegedly 10 nM), undiluted. As you can see, it's fairly high-MW (460 bp), so smaller libraries might cluster preferentially, and it's actually 8 nM.
Attached Files
File Type: pdf phiX_bioanalyzer.pdf (173.9 KB, 98 views)
jwfoley is offline   Reply With Quote
Old 08-25-2015, 09:04 PM   #2
jlei_face
Member
 
Location: San Diego, CA

Join Date: Oct 2014
Posts: 10
Default

Thanks for sharing this data.
I'd just like to add that concentrations of PhiX vary from lot to lot so 8nM wouldn't necessarily be applicable to every tube.
jlei_face is offline   Reply With Quote
Old 05-31-2017, 06:26 AM   #3
ritujasaxena
Junior Member
 
Location: India

Join Date: Apr 2016
Posts: 1
Default

Thanks for the info..

Yes the concentration varies a lot.

I have observed it to be between 8-14 nM in different cases.
ritujasaxena is offline   Reply With Quote
Reply

Tags
bioanalyzer, phix, pooling

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 06:47 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO