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02-21-2013, 02:07 PM
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#1 |
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Junior Member
Location: Chicago Join Date: Feb 2013
Posts: 1
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Genomic version of PhiX?
Basically my lab's looking for a version Phix that has not been sheered, doesn't have any adaptors or barcodes on it, etc., etc. to use as a in-line control for our library preparations.
Any of know of anything like that? |
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02-21-2013, 02:41 PM
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#2 |
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Senior Member
Location: USA Join Date: Jul 2012
Posts: 185
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You can buy PhiX 174 genomic DNA from any vendor that sells DNA. However, I would advise against using it as an inline control as it would contaminate your sequencing data. You could use it as a separate positive control, although any genomic DNA would could be used for this purpose. The Illumina in-line controls would be the ideal way to monitor the library preparation process.
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02-21-2013, 09:24 PM
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#3 |
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--Site Admin--
Location: SF Bay Area, CA, USA Join Date: Oct 2007
Posts: 1,358
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