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Old 02-21-2013, 02:07 PM   #1
stephm524
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Location: Chicago

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Post Genomic version of PhiX?

Basically my lab's looking for a version Phix that has not been sheered, doesn't have any adaptors or barcodes on it, etc., etc. to use as a in-line control for our library preparations.

Any of know of anything like that?
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Old 02-21-2013, 02:41 PM   #2
kcchan
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You can buy PhiX 174 genomic DNA from any vendor that sells DNA. However, I would advise against using it as an inline control as it would contaminate your sequencing data. You could use it as a separate positive control, although any genomic DNA would could be used for this purpose. The Illumina in-line controls would be the ideal way to monitor the library preparation process.
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Old 02-21-2013, 09:24 PM   #3
ECO
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How hard have you looked?

https://www.neb.com/search?q=φX174
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