I have been searching for for the overhang sequence on the i7 and i5 indices of the Truseq Custom amplicon Index kit, but couldn't find them..
We have designed about 10 primer pairs (microbiology, fungal and bacterial) and we want to link these primers, through two step pcr, to the indices of the Truseq amplicon kit (which is part of the Cancer Panel Kit from Illumina). Illumina won’t provide them since they are classified/company secret..
So far I only found information about adapters of Nextera and Truseq DNA kits.
Any help could be usefull.
We have designed about 10 primer pairs (microbiology, fungal and bacterial) and we want to link these primers, through two step pcr, to the indices of the Truseq amplicon kit (which is part of the Cancer Panel Kit from Illumina). Illumina won’t provide them since they are classified/company secret..
So far I only found information about adapters of Nextera and Truseq DNA kits.
Any help could be usefull.
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