SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Agilent human exon coverage plots SMO Genomic Resequencing 9 11-25-2012 06:14 AM
SureSelect Human All Exon V4 and V4+UTRs luckylove General 0 07-30-2012 01:17 AM
Exon Sequencing of Human Chromosome X: Im in serious trouble frossit Genomic Resequencing 2 10-13-2011 05:07 AM
PubMed: Differential genome-wide profiling of tandem 3' UTRs among human breast cance Newsbot! Literature Watch 0 09-07-2011 02:00 AM
Human exon, intron and promotor bedfile khb Bioinformatics 1 12-22-2010 05:47 AM

Reply
 
Thread Tools
Old 04-08-2015, 02:32 PM   #1
SLK
Junior Member
 
Location: AZ

Join Date: Apr 2015
Posts: 1
Default SureSelectXT Human All Exon V5+UTRs

Please see attached image. I am wondering what is and what causes that minor bump at 700-1500 bp. Is that concatemer?

# of PCR cycle : 10

Thanks
Attached Images
File Type: png Capture.PNG (80.4 KB, 17 views)
SLK is offline   Reply With Quote
Old 04-08-2015, 10:49 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,234
Default

It is most likely result of over-amplification. I guess you have used less input DNA than recommended.
nucacidhunter is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 12:42 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO