Any aftermarket 454 reagents being (or going to be) produced?

hoytpr · 05-28-2015, 08:31 AM

So when the Roche 454 reagents dry up, will no other company step up with compatible kits? I notice Epicentre has dropped all their Nextera stuff.

Will Roche make the reagents public in case we want to try and make our own kits? We can pretty much guess at a lot of the protocols, but it would be great to just have a cookbook method available.

I asked about "repurposing" the 454-Junior we have, but no ideas so far.

mps4208 · 05-29-2015, 05:04 AM

I haven't heard anything yet, but we are hoping the same thing.

Epicentre is slowly shifting everything to Illumina, at least Illumina picked up the Nextera.

kmcarr · 05-29-2015, 04:27 PM

Reagents are one thing, but I can't imagine anyone would want to lay out for manufacturing picotiter plates.

hoytpr · 06-01-2015, 08:11 AM

We can get the picotitre plates pretty clean with washing and sonicating. I know labs that have reused them. Also, the channel glass they are made from has been around for a couple decades now (before 454 technology) and will still be made.

martin2 · 06-17-2015, 08:34 AM

Quote:

Originally Posted by hoytpr

We can get the picotitre plates pretty clean with washing and sonicating. I know labs that have reused them.

And that is exactly what ruined a number of projects. I spent insane amount of time cleaning up contaminated projects. Don't do it. Although some labs could be more thorough in sonication and water exchange, clearly the beads not only do remain in the PTP plate but even the enzymes keep working in a following sequencing. Feel free to place an order on my website www.bioinformatics.cz for cleanup of your current data. ;-)

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05-28-2015, 08:31 AM	#1
hoytpr Member Location: Stillwater Join Date: Dec 2009 Posts: 62	Any aftermarket 454 reagents being (or going to be) produced? So when the Roche 454 reagents dry up, will no other company step up with compatible kits? I notice Epicentre has dropped all their Nextera stuff. Will Roche make the reagents public in case we want to try and make our own kits? We can pretty much guess at a lot of the protocols, but it would be great to just have a cookbook method available. I asked about "repurposing" the 454-Junior we have, but no ideas so far.

05-29-2015, 05:04 AM	#2
mps4208 Member Location: Chapel Hill, NC Join Date: Dec 2012 Posts: 12	I haven't heard anything yet, but we are hoping the same thing. Epicentre is slowly shifting everything to Illumina, at least Illumina picked up the Nextera.

05-29-2015, 04:27 PM	#3
kmcarr Senior Member Location: USA, Midwest Join Date: May 2008 Posts: 1,172	Reagents are one thing, but I can't imagine anyone would want to lay out for manufacturing picotiter plates.

06-01-2015, 08:11 AM	#4
hoytpr Member Location: Stillwater Join Date: Dec 2009 Posts: 62	We can get the picotitre plates pretty clean with washing and sonicating. I know labs that have reused them. Also, the channel glass they are made from has been around for a couple decades now (before 454 technology) and will still be made.