Ion PI™ Controls usage with Ion Torrent PGM

Alex Alexl · 07-09-2019, 06:44 AM

Hello,
Does anybody know about compatibility between control sphere from Ion PI™ Controls 200 Kit and PGM in terms of sphere size, sequencing primer and so on? I need a control sphere for PGM but it looks like there is just Ion PI control kit with premade libraries and spheres. Is there any other control sphere kit for PGM?

r.rosati · 07-09-2019, 02:04 PM

Hi,
you're right, the v3 version of the Ion PGM™ Controls Kit doesn't have any Control Ion Spheres. The v2 version did.
Well size is notably bigger in PGM chips compared to Proton's PI, so I would say that the ISPs are not interchangeable, unfortunately. You might end up losing them during the run, I suppose.
An aliquot of Control ISPs comes with PGM sequencing kits - although they are barely more than what's needed for the kit's normal usage.
Did you need to make a test run with only control ISPs, as opposed to the normal amount of control ISPs that go in a sequencing run? Thermo engineers do use full control runs to check the machines at installation, so perhaps Thermo might be able to help?

Alex Alexl · 07-10-2019, 01:53 AM

Thanks a lot. Yes I need to run full chip with control IPS.

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Ion Torrent PGM vs Illumina MiSeq	ramujana	Ion Torrent	121	01-05-2016 10:06 AM
Ion Torrent PGM Analysis Pipeline	byou678	Ion Torrent	5	11-20-2015 03:18 AM
Difference between Ion torrent proton and Ion PGM	Bionerd	Ion Torrent	7	09-30-2015 02:55 PM
Ion Torrent PGM multiplexing	eosin	Ion Torrent	7	03-29-2012 06:00 PM

07-09-2019, 06:44 AM	#1
Alex Alexl Junior Member Location: New York Join Date: Jul 2019 Posts: 2	Ion PI™ Controls usage with Ion Torrent PGM Hello, Does anybody know about compatibility between control sphere from Ion PI™ Controls 200 Kit and PGM in terms of sphere size, sequencing primer and so on? I need a control sphere for PGM but it looks like there is just Ion PI control kit with premade libraries and spheres. Is there any other control sphere kit for PGM?

07-09-2019, 02:04 PM	#2
r.rosati Member Location: Brazil Join Date: Aug 2015 Posts: 91	Hi, you're right, the v3 version of the Ion PGM™ Controls Kit doesn't have any Control Ion Spheres. The v2 version did. Well size is notably bigger in PGM chips compared to Proton's PI, so I would say that the ISPs are not interchangeable, unfortunately. You might end up losing them during the run, I suppose. An aliquot of Control ISPs comes with PGM sequencing kits - although they are barely more than what's needed for the kit's normal usage. Did you need to make a test run with only control ISPs, as opposed to the normal amount of control ISPs that go in a sequencing run? Thermo engineers do use full control runs to check the machines at installation, so perhaps Thermo might be able to help?

07-10-2019, 01:53 AM	#3
Alex Alexl Junior Member Location: New York Join Date: Jul 2019 Posts: 2	Thanks a lot. Yes I need to run full chip with control IPS.