I used Tophat> Cufflink>Cuffcomare>Cuffdiff two comare two samples (treated and control, no replicate) for RNAseq analysis to study differential expression. The output files of CuffDiff does not give any output- just of row of title for promoter Diff., CDS, splicing diff, FRKM TSS group CDS FRKM. The data in the output is only for gene expression.
How is that possible? Am I doing something wrong or it is the way the output will be?
Any suggestion.
Thanks.
How is that possible? Am I doing something wrong or it is the way the output will be?
Any suggestion.
Thanks.
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