Hello everyone,
I am producing Ion Torrent sequencing libraries both using the ligation based library kit and the ampliseq strategy. The only adaptor sequences I can find from Lifetech are the ones involved in ampliseq:
Forward primer (Primer A-key):
5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-template-specific-sequence-3’
Reverse primer (Primer P1-key):
5’-CCTCTCTATGGGCAGTCGGTGAT-template-specific-sequence-3’
For quantitation purposes I use the Taqman based qPCR kit. This is however only possible with the ligated libraries. On this basis I assume the double stranded oligos used for ligation contains a longer sequence, than the one generated in the ampliseq PCR.
Does anyone have this sequence of the ligation adaptors?
I know this may well, for some obscure reason, be one of Lifetechs little secrets and that I may have to go through the trouble of cloning and sequencing the adaptors myself.
I am producing Ion Torrent sequencing libraries both using the ligation based library kit and the ampliseq strategy. The only adaptor sequences I can find from Lifetech are the ones involved in ampliseq:
Forward primer (Primer A-key):
5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-template-specific-sequence-3’
Reverse primer (Primer P1-key):
5’-CCTCTCTATGGGCAGTCGGTGAT-template-specific-sequence-3’
For quantitation purposes I use the Taqman based qPCR kit. This is however only possible with the ligated libraries. On this basis I assume the double stranded oligos used for ligation contains a longer sequence, than the one generated in the ampliseq PCR.
Does anyone have this sequence of the ligation adaptors?
I know this may well, for some obscure reason, be one of Lifetechs little secrets and that I may have to go through the trouble of cloning and sequencing the adaptors myself.
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