Hello all,
heading just a quick reply
I aligned my color-space fastq by bowtie 1 and got sam as output. But in my all mapped reads i have 255 instead any quality information, that means quality information is absent. However, my question is..
1) Why its happened ??
2) Lacking this quality information would really hurt for my further analysis by this sam ??? (lets say DE analysis or any downstream analysis )
Thanks in advance
heading just a quick reply
I aligned my color-space fastq by bowtie 1 and got sam as output. But in my all mapped reads i have 255 instead any quality information, that means quality information is absent. However, my question is..
1) Why its happened ??
2) Lacking this quality information would really hurt for my further analysis by this sam ??? (lets say DE analysis or any downstream analysis )
Thanks in advance
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