Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • GALAXY: Huge amount of data?

    Hi all,

    I have a (probably) very naive question ..

    We are running a few machines here as a core facility (Hiseq2000, GAIIx, 454tit,SOLiD) ... there are a couple of users interested in the capabilities of GALAXY, so I am thinking about a local install of the whole package.

    My concern is the data amount. E.g. one Hiseq2000 lane with a PE whole exome lib is roughly 2x20G (unzipped) fastq. I can definitively reduce the amount by zipping the datasets. But this is still a huge amount of data to be uploaded via Browser ...
    How is this working in practice if you have more than one lane?
    How are the jobs scheduled?

    I probably need to read more en detail before starting to set up my own installation ...

    thanks for any comment ..

    cheers,
    Sven

  • #2
    Regarding the upload problem: Galaxy can be setup to let users upload their files by FTP, however, in your situation as a core facility you can import the files directly into Galaxy from disk. People do this as part of an automatic sequencing service pipeline - have a search on the Galaxy mailing list.

    Regarding the general data volume problem: Galaxy by default keeps all the files on disk, and you can have cron jobs to clean up "deleted" datasets. Some users on the Galaxy mailing list have reported needing to be more aggressive on their server to avoid running out of space.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin


      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
      Yesterday, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    55 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    52 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    45 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    55 views
    0 likes
    Last Post seqadmin  
    Working...
    X