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Old 07-16-2020, 12:41 AM   #1
Punty
Junior Member
 
Location: Germany

Join Date: Jul 2020
Posts: 1
Question How to distribute samples into multiple libraries

Dear community,

I am complete newbie to all of this; I apologize for my glaring na´vetÚ in advance.

My issue: I do not know how to distribute my RNA samples into three libraries such that they are equally balanced and unbiased for RNAseq.

My RNA samples are from patient biopsies obtained from multiple institutions, extracted on different days, and from a heterogeneous patient population.

I have multiple variables that I would like to balance between the three libraries, including, but not limited to:

-Disease State
-Disease Score
-Patient Age
-Patient Sex
-Clinic

Additionally, I would also like to balance the libraries in terms of RNA quality (RIN), date of extraction, and who did the extraction...

Overall, my goal is to get three libraries that are equally weighted for everyone of those factors I listed (and likely more...). Is this a good strategy? And if so, how would you go about doing this?

Thank you for any tips or resources!

-Punty
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Old 07-16-2020, 07:31 AM   #2
GenoMax
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Location: East Coast USA

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Posts: 7,077
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I don't fully understand what you are asking but is this with reference to downstream data analysis (factors to be added in model). Is that correct?
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