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Old 05-01-2012, 06:14 AM   #1
Location: miami

Join Date: Aug 2011
Posts: 10
Default pre-small RNA library prep

I am attempting to prep a small rna library and have heard of people selecting for the small rna prior to introduction into protocols. I was wondering if anyone could elaborate on these methods or kits. particularly, I am looking for a method that doesn't involve an acrylamide gel. we have done preps before but tend to get more mRNA and tRNA reads than we would like using the Illumina smRNA kit.

Thanks for any advice that can be given.
chaos81 is offline   Reply With Quote
Old 05-03-2012, 06:23 AM   #2
Location: UK

Join Date: Oct 2010
Posts: 21

I have tried the MirVana Kit from Ambion/LifeTech. That works well and gives you everything below 200nt.
mboth is offline   Reply With Quote

illumina hiseq, library construction, mirna library, small rna sequencing

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