SEQanswers

Go Back   SEQanswers > Literature Watch



Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Single Molecule Sequencing with a HeliScope Genetic Analysis System. Newsbot! Literature Watch 0 10-05-2010 02:00 AM
Question of small quantity of cell or single cell sequencing hxygz Sample Prep / Library Generation 1 07-16-2009 11:48 PM
mRNA-Seq whole-transcriptome analysis of a single cell. benimmyeo Literature Watch 0 04-26-2009 11:12 PM
Second SOLiD Publication: Stem cell transcriptome profiling ECO SOLiD 0 06-03-2008 08:29 AM
PubMed: High-Throughput Single Copy DNA Amplification and Cell Analysis in Engineered Newsbot! Literature Watch 0 04-16-2008 07:51 AM

Reply
 
Thread Tools
Old 12-02-2009, 03:44 PM   #1
GeeSharpMinor
Junior Member
 
Location: washington

Join Date: Mar 2009
Posts: 4
Default mRNA-Sequencing Whole Transcriptome Analysis of a Single Cell on the SOLiD System

mRNA-Sequencing Whole Transcriptome Analysis of a Single Cell on the SOLiD System.
Lao KQ, Tang F, Barbacioru C, Wang Y, Nordman E, Lee C, Xu N, Wang X, Tuch B, Bodeau J, Siddiqui A, Surani MA.

J Biomol Tech. 2009 Dec;20(5):266-71.

We have developed a sequencing-based gene expression profiling assay at single-cell resolution by combining a modified single-cell whole transcriptome amplification method with the next generation sequencing technique, the SOLiD system. Using this assay, we have shown that blastomeres in a four-cell stage embryo have similar gene expression, which is compatible with the fact that they have similar developmental potential. We proved that compared with cDNA microarray technique, our single-cell cDNA SOLiD sequencing assay can detect expression of thousands of more genes. Moreover, for the genes detected by microarray and SOLiD sequencing, our assay detected new transcript variants for a large proportion of them, which confirms unambiguously at single-cell resolution that the transcriptome complexity is higher than expected traditionally. Finally, by using our assay to Dicer knockout (KO) and Ago2 KO oocytes, we showed that a significant amount of transposons were up-regulated abnormally in Dicer/Ago2 KO mature oocytes compared with wild-type controls.

http://www.ncbi.nlm.nih.gov/pubmed/19949700/
GeeSharpMinor is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 12:40 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO