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Old 11-06-2013, 10:45 PM   #1
Location: haifa israel

Join Date: Jun 2011
Posts: 62
Default Werid RNA traces- bump above 28S - can I make libraries from this?

Hi all,
I have traces from a fragment analyzer (very similar to bioanalyzer; basically your capillary gel run) and am getting some weird traces. Some (well A2, A6 and A7 for example) have things higher than 28S - any idea what these can be? Also - A6 and A7 have very streange humps instead of just two distinct peaks. Can I make libraries out of these?
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File Type: pdf samples SW4_13 thru 18, and SW4-4.pdf (192.4 KB, 42 views)
Noa is offline   Reply With Quote
Old 11-08-2013, 12:37 PM   #2
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Location: San Diego

Join Date: Feb 2012
Posts: 25

Hi Noa-

My guess is genomic DNA contamination. Did you DNase treat it when on the column?
JeremyDay is offline   Reply With Quote

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