Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Ion Torrent reverse primer - P1 or B Retro Ion Torrent 9 03-12-2012 11:13 AM
Ion torrent blog on raw accuracy - good primer for accuracy grand challenge lek2k Ion Torrent 3 09-20-2011 12:13 AM
Can smRNA sequencing primer and genomic DNA sequencing primer mixed in one lane? whimsy Illumina/Solexa 3 04-06-2011 09:30 AM
who know extract linker/Primer sequences for HTS sequencing? feng Bioinformatics 2 10-26-2010 02:19 PM
Going from biotinylated primer to unlabeled primer in emPCR chunnan20451 454 Pyrosequencing 0 09-01-2010 05:22 PM

Thread Tools
Old 03-26-2014, 02:44 AM   #1
Junior Member
Location: Utrecht

Join Date: Mar 2014
Posts: 1
Default Primer sequences emPCR and sequencing primer Ion Torrent


Does anyone know the exact sequences of the primers that are used for the clonal amplification with the Ion PGM™ Template OT2 400 Kit? In addition I like to know what the sequence is of the sequencing primer used in the Ion PGM™ Sequencing 400 Kit?
I like to know exactly where the primers bind to the A and P1 adaptors.
laurakrol is offline   Reply With Quote
Old 04-25-2014, 04:08 AM   #2
Junior Member
Location: BRAZIL

Join Date: Apr 2014
Posts: 2

Laura, there's a good tech note about it (attached) and also this answer at "The sequences can also be found on page 68 of the Ion Xpress™ Plus gDNA Fragment Library Preparation user guide. - See more at:"
Attached Files
File Type: pdf ION_Amplicon sequencing.pdf (1.14 MB, 168 views)
Fernanda is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:19 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO