Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Is there a but in BWA 3'-end trimming?

    I got the following lines in the BAM file produced from BWA mapping using -q 6. It seems that the soft clipping in the CIGAR field is reporting one base less than should have been trimmed by the BWA trimming algorithm.

    Code:
    ILLUMINA-8C38E9_0111:3:99:15568:1908#0  83      1       9999977 60      32S50M  =       9999814 -213    GGCAGGGAGGTCCCTTGGGCCCAGGGTTTCCAGACCAGCCTGGCCGACACGGCGAAACCCCGTCTCTACAATAAATTAAAAT      #################################?:<?-?:445>-CA:@A??CDADA?=?BDEDEDEAEEEEEBEABEDE-E
    ILLUMINA-8C38E9_0111:7:46:16510:9079#0  83      1       9999978 60      5S77M   =       9999740 -315    TACAGCCCAGCCTGGCCGACACGGCGAAACCCCGTCTCTACAATAAATTAAAATATTAGCTGGGCATGGTGGTGTGTGCTTG      ######?=C=CC@FDFEGBGEGGGGGFDBGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGFGGGGGGGGGGEG
    ILLUMINA-8C38E9_0111:7:86:18250:3356#0  147     1       9999985 60      33S49M  =       9999455 -579    AGACCGTGTGACACCGGGAGTAACAGCCCAGCCGGGCCGACACGGCGAAACCCCGTCTCTACAATAAATTAAAATATTAGCT      ##################################B?CFFEFDGGGGGGGEGGGFGGGGFFGGGGGGFGFFGGGGGGGGGGGG
    ILLUMINA-8C38E9_0111:3:37:15542:13225#0 99      1       10000237        50      71M11S  =       10000385        230     GTTTTTTGTTTGTTTTGAGACAGAGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGAGCAATCTCAGCTCACTGCAAGCC      GGDGGGGGGEDFGGGF@GGGGGGEGEGGGGGED?GDFEED>)=:5-662.0<:<5749.<81682:@?9@############
    ILLUMINA-8C38E9_0111:3:53:3839:8121#0   99      1       10000270        51      81M1S   =       10000541        353     TGTCGCCCAGGCTGGAGTGCAGTGGAGCAATCTCAGCTCACTGCAAGCTCCGCCTCCCGGGCTCACACCATTCTCCTGCCCC      GGGGEGGGGGDGGFGGGEGGEFDFFFD?FFDEGGFDFFGGGGFGGGEABEEEDEACBE7-6%-23BABA5,:;;=?0-??##
    Could somebody check your own BWA mapped BAM files to see if this is a general bug in BWA? Or is this a generally known feature?!

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    Yesterday, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
56 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
52 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
45 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
55 views
0 likes
Last Post seqadmin  
Working...
X