I am a MS student working with soil microbiome. I am looking for the presence of a gene family in soils under certain conditions and would like to use RNASeq to profile its expression in soil community. Gene is conserved in bacteria and fungi, so I will be analyzing the transcriptomes of both. Illumina HiSeq is off the table, but Im looking into Illumina MiSeq, IonTorrent, or Roche 454. Anyone have any insight?
Thanks
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