I recently had a MiSeq run fail and am not 100% sure what caused it.
Here are some details:
Amplicon sequencing run of RNA FFPE samples (125 genes) using a MiSeq V3 (600 cycle) kit loaded at 14 pM. Cluster Density is 909 K/mm2.
Phix spiked in at 15% and sequencing run configured to 131 x 2 paired-end.
SAV Data by Cycle, %Q30: https://imgur.com/a/NZf7Gfb
SAV Run summary: https://imgur.com/a/nQa3sEa
I am leaning towards reagent failure, incorrect run parameters or an issue with the indices.
I would appreciate any help.
Thanks!
Here are some details:
Amplicon sequencing run of RNA FFPE samples (125 genes) using a MiSeq V3 (600 cycle) kit loaded at 14 pM. Cluster Density is 909 K/mm2.
Phix spiked in at 15% and sequencing run configured to 131 x 2 paired-end.
SAV Data by Cycle, %Q30: https://imgur.com/a/NZf7Gfb
SAV Run summary: https://imgur.com/a/nQa3sEa
I am leaning towards reagent failure, incorrect run parameters or an issue with the indices.
I would appreciate any help.
Thanks!
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