Hi,all
I assembled a fungal transcriptome by Trinity and I want to get orf or cds sequence from them.
when I aligned the sequences from cDNA by PCR to the orf predicted from the transcripts by a script in Trinity, I found that the predicted orf was not accurate.
So, I want to get advice how to predict fungal orf from assembled transcritome accurate.
I assembled a fungal transcriptome by Trinity and I want to get orf or cds sequence from them.
when I aligned the sequences from cDNA by PCR to the orf predicted from the transcripts by a script in Trinity, I found that the predicted orf was not accurate.
So, I want to get advice how to predict fungal orf from assembled transcritome accurate.