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  • Illumina Hiseq data analysis

    Dear All:

    I am just starting to deal with Metagenomic sequences. I got some previous data from others with illumina Hiseq paired end. I am looking for some software or pipeline to help me with analyzing. Any good suggestion with it?
    I looked at MG-RAST, but as I got a very large data ( 16 Gb), is it fine to upload?
    I was also looked at QIIME which I was using for 454 before, does QIIME handle with metagenomic data?
    Or what other good software can be used?
    Thank you for your help.

  • #2
    Tools choice is depending on what you are looking as answers for biological questions.
    For metagenomes analysis, I think, MG-RAST, RAMMCAP are best the tools to start for beginners in the goal to understand how to proceed the exploration of Organism/OTU/Phylotype abundance and Functional abundance. Hope this comment is useful
    __Bach__

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