Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Canu error: 'failed to create the overlap store'

    Trying to run the following command:

    $ canu -trim -p PacBio10_trimmed -d PacBio10_trimmed genomeSize=200000 -pacbio-corrected /work/03868/lguzmanz/liz_trials/PacBio_correctedreads/corrected_Canu_pacbio10.correctedReads.fasta.gz "useGrid=false"

    Got the following error message:


    ERROR:
    ERROR: Failed with exit code 1.

    ERROR:

    Don't panic, but a mostly harmless error occurred and canu failed.

    Disk space available: 17025761.9 GB

    Last 50 lines of the relevant log file (/work/03868/lguzmanz/liz_trials/april_20/PacBio10_trimmed/trimming/PacBio10_trimmed.ovlStore.err):

    Summing overlap counts for 1 reads from '/work/03868/lguzmanz/liz_trials/april_20/PacBio10_trimmed/trimming/1-overlapper/001/000001.counts'.
    Found no overlaps to sort.

    canu failed with 'failed to create the overlap store'.


    I had to manually correct 18 PacBio reads because the correct/trim/assemble default canu code ran into issues, now that I look back on it, it was probably the same error with the "failed to create overlap store" when trimming.

    Any help would be greatly appreciated!

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin


    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
    Yesterday, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
40 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
41 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
36 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
55 views
0 likes
Last Post seqadmin  
Working...
X