I have Illumina short read, 2X150bp right now, around 12,3509 Gb data.
I just curious whether got any parameter or formula able to calculate the minimum short read required to assemble a transcript sequence by transcriptome assembler program in order to obtain comprehensive transcript?
eg. must have at least 1Mb Illumina short read in order to assemble it.
Do we need consider coverage and depth of data when determine or calculate the minimum short read required for transcriptome assembly as well?
Thank you!
I just curious whether got any parameter or formula able to calculate the minimum short read required to assemble a transcript sequence by transcriptome assembler program in order to obtain comprehensive transcript?
eg. must have at least 1Mb Illumina short read in order to assemble it.
Do we need consider coverage and depth of data when determine or calculate the minimum short read required for transcriptome assembly as well?
Thank you!
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