Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • SSPACE scaffolding

    Hi All,

    I am using SSPACE for scaffolding. After running sspace i am getting same no of scaffolds as the no of contigs i got but the no of scaffold should be less then the no of contig. Am i correct?

    Here is my command

    perl SSPACE_Standard_v3.0.pl -l libraries.txt -s contigs.fa -T 4 -b out

    The summary file

    READING READS lib1:
    ------------------------------------------------------------
    Total inserted pairs = 2880237
    ------------------------------------------------------------



    LIBRARY lib1 STATS:
    ################################################################################

    MAPPING READS TO CONTIGS:
    ------------------------------------------------------------
    Number of single reads found on contigs = 209
    Number of read-pairs used for pairing contigs / total pairs = 33 / 33
    ------------------------------------------------------------

    READ PAIRS STATS:
    Assembled pairs: 33 (66 sequences)
    Satisfied in distance/logic within contigs (i.e. -> <-, distance on target: 3000 +/-750): 0
    Unsatisfied in distance within contigs (i.e. distance out-of-bounds): 0
    Unsatisfied pairing logic within contigs (i.e. illogical pairing ->->, <-<- or <-->): 0
    ---
    Satisfied in distance/logic within a given contig pair (pre-scaffold): 0
    Unsatisfied in distance within a given contig pair (i.e. calculated distances out-of-bounds): 33
    ---
    Total satisfied: 0 unsatisfied: 33


    Estimated insert size statistics (based on 0 pairs):
    Mean insert size = 0
    Median insert size = 0

    REPEATS:
    Number of repeated edges = 0
    ------------------------------------------------------------

    ################################################################################

    SUMMARY:
    ------------------------------------------------------------
    Inserted contig file;
    Total number of contigs = 1058
    Sum (bp) = 35688531
    Total number of N's = 0
    Sum (bp) no N's = 35688531
    GC Content = 45.29%
    Max contig size = 382053
    Min contig size = 300
    Average contig size = 33732
    N25 = 162114
    N50 = 88961
    N75 = 48154

    After scaffolding lib1:
    Total number of scaffolds = 1058
    Sum (bp) = 35688531
    Total number of N's = 0
    Sum (bp) no N's = 35688531
    GC Content = 45.39%
    Max scaffold size = 382053
    Min scaffold size = 300
    Average scaffold size = 33732
    N25 = 162114
    N50 = 88961
    N75 = 48154

    ------------------------------------------------------------

    Can anyone help me to understand why it is coming like this?

    Any help will be appreciate..

    Thanks in advance....

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
59 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
57 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
51 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
55 views
0 likes
Last Post seqadmin  
Working...
X