When I use poreminion fragstats to classify fast5's, theoretically I could use the 4th and 5th field to classify reads into 1D template-only, 1D complement-only, normal 2D and full 2D. In reality, after working with five datasets I downloaded from ENA, none of them contains any 1D complement-only reads.
I also wrote a simple python script to count template events and complement events but I never saw any reads that have zero template events but a positive number of complement events.
Does that mean 1D complement-only read doesn't exist???
I also wrote a simple python script to count template events and complement events but I never saw any reads that have zero template events but a positive number of complement events.
Does that mean 1D complement-only read doesn't exist???
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