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Hi all,
I used TruSeq stranded total RNA sample preparation guide to remove ribosomal RNA. However, my final library had a high peak in bio-analyzer data (as attached file).
My condition list below:
RIN of RNA: 10
input RNA: 1 ug
digest time: 8 mins as protocol
another condition was by the protocol.
Could anyone help me to figure out which step I should modify?
Many thanks.
Aero Ma
I used TruSeq stranded total RNA sample preparation guide to remove ribosomal RNA. However, my final library had a high peak in bio-analyzer data (as attached file).
My condition list below:
RIN of RNA: 10
input RNA: 1 ug
digest time: 8 mins as protocol
another condition was by the protocol.
Could anyone help me to figure out which step I should modify?
Many thanks.
Aero Ma