targets of exon enrichment probe

[johnshembb]

Hi,

I am just wondering whether the target regions of exon enrichment kits currently available focus on exact the exons only or include some up- and/or down stream sequence of a gene?

Really appreciate if someone happen to know this

Thanks

[NextGenSeq]

You get about 50 to 100 bases of flanking intron sequence in virtually every kit.

[johnshembb]

You get about 50 to 100 bases of flanking intron sequence in virtually every kit.

Thank you for your response!

I have another question about the illumina TruSeq kit. Do you know what the "Ref exons plus 0" means?

Thanks!