Hello Everyone,
I am new person to RNA Seq work.I made libraries from FFPE RNA as per protocol.RNA input was from 0.5-1ug for different samples.
But i can hardly see any yield on bioanalyzer and nanodrop readings are also 10ng/ul or below.
This is disappointing for me.I have been careful.I don't know where i lost my nucleic acid.One thing which i can remember about Ribosomal RNA removal beads step ,those beads were really difficult to dissolve.
Any suggestions will be welcomed.
Thanks
I am new person to RNA Seq work.I made libraries from FFPE RNA as per protocol.RNA input was from 0.5-1ug for different samples.
But i can hardly see any yield on bioanalyzer and nanodrop readings are also 10ng/ul or below.
This is disappointing for me.I have been careful.I don't know where i lost my nucleic acid.One thing which i can remember about Ribosomal RNA removal beads step ,those beads were really difficult to dissolve.
Any suggestions will be welcomed.
Thanks
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