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Old 04-18-2012, 06:40 AM   #1
cwachtel
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Location: Israel

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Default Diluting Library for One Touch

Hi Everyone,

I recently prepared three libraries, and after determining the dilution factors I did emPCR reactions using the one touch on 2 of them. Neither of them seem to have worked based on Qubit results (65% and 115% templated ISPs). I am not sure if I am adding too much of my library or not enough. Does anyone have any experience with this?

Thanks

Chaim
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Old 04-23-2012, 01:33 PM   #2
snetmcom
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you are adding too much if this is before enrichement

Last edited by snetmcom; 04-26-2012 at 04:07 PM.
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emulsion pcr, ion torrent, one touch, template prep

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