Hello,
I have received a matrix with normalized values from an RNA sequencing experiment; the normalization was done as following: The raw counts were converted to cpm and then to RLE by using edgeR. I have no other information, only the matrix with these values.
How would you continue in order to do a DE analysis?
Would it make sense to try to convert them to raw counts?
Some help would be appreciated!
I have received a matrix with normalized values from an RNA sequencing experiment; the normalization was done as following: The raw counts were converted to cpm and then to RLE by using edgeR. I have no other information, only the matrix with these values.
How would you continue in order to do a DE analysis?
Would it make sense to try to convert them to raw counts?
Some help would be appreciated!
Comment