Any programs for analyzing solexa transcriptome (alternative splicing) ?
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
I wonder if Solexa reads, say 50bp can identify alternative splicing when you don't have a reference genome. How can we tell the alignment software to map the reads so we can find splice variant?
Alternative splicing has been reported using 454 reads. The longer reads is probably an advantage. Anyone has done similar work using Solexa reads?
Comment
-
i have reads.fq (5 000 000 reads RNA-seq) genome.fa
when i use bowtie align the reads, it aligns 4 200 000 reads.
but when i use tophat align the reads, the output file accepted_hits.sam only contains 1 900 000 reads. junctions.bed file is blank.
Comment
-
Originally posted by baohua100 View PostAny programs for analyzing solexa transcriptome (alternative splicing) ?
With a reference transcriptome, if you want to assign a quantity to each splicing variant I only know about this flux capacitor. Maybe not fully stable yet. I'm sure a lot of similar projects are in progress.
(Disclaimer: I personally know these people, they are friends of mine -but don't worry, contrary to me they still work in academic research).
Much more is expected to come from the RGASP project. I'd suggest to keep an eye on this.
Then, there are commercial products that claim to address "alternative splicing", but I am curious about what they really do...
yours,
s.
Comment
Latest Articles
Collapse
-
by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
Channel: Articles
04-04-2024, 04:25 PM -
-
by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
Channel: Articles
03-22-2024, 06:39 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 04-11-2024, 12:08 PM
|
0 responses
24 views
0 likes
|
Last Post
by seqadmin
04-11-2024, 12:08 PM
|
||
Started by seqadmin, 04-10-2024, 10:19 PM
|
0 responses
25 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 10:19 PM
|
||
Started by seqadmin, 04-10-2024, 09:21 AM
|
0 responses
21 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 09:21 AM
|
||
Started by seqadmin, 04-04-2024, 09:00 AM
|
0 responses
52 views
0 likes
|
Last Post
by seqadmin
04-04-2024, 09:00 AM
|
Comment