Hi all,
I have a question about the accuracy of the Illumina SRA ladder (20nt-100nt) that is used when making small RNA libraries.
I have been running the microRNA marker from NEB (#N2102S) next to the Illumina SRA ladder and I am getting some weird results: the 17nt band is migrating slower than (or at least equal to) the 20nt band of the SRA marker, the 21nt band is sitting well into the twenties and the 25nt band of the NEB marker is just shy of 30nt compared to the SRA ladder. The migration pattern is quite reproducible, even after ethanol precipitating the NEB marker and loading it in on the gel using Illumina's own loading buffer.
I think one of these two markers must be migrating at the wrong rate.
Has anyone had a similar experiences with either of these markers?
Cheers
I have a question about the accuracy of the Illumina SRA ladder (20nt-100nt) that is used when making small RNA libraries.
I have been running the microRNA marker from NEB (#N2102S) next to the Illumina SRA ladder and I am getting some weird results: the 17nt band is migrating slower than (or at least equal to) the 20nt band of the SRA marker, the 21nt band is sitting well into the twenties and the 25nt band of the NEB marker is just shy of 30nt compared to the SRA ladder. The migration pattern is quite reproducible, even after ethanol precipitating the NEB marker and loading it in on the gel using Illumina's own loading buffer.
I think one of these two markers must be migrating at the wrong rate.
Has anyone had a similar experiences with either of these markers?
Cheers
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