Hello everyone,
I processed 2 C.elegans samples with Nextera XT protocol (Miseq), and before i continue to beads normalization i checked the non-normalized samples on Bioanalyzer (attached image). I didn't proceed to the normalization step, because i had some doubts about the sequencing performance in this kind of samples, since i have only peak after 400-500 bp.
I checked the starting amount, I quantified (Qubit) and I didn't load more than 1ng.
The tagmentation step was like that in the manual (55ºC > 5min).
Any idea from what could influenced the tagmentation?
If it was you, did you continued to sequence?
Thanks in advance
I processed 2 C.elegans samples with Nextera XT protocol (Miseq), and before i continue to beads normalization i checked the non-normalized samples on Bioanalyzer (attached image). I didn't proceed to the normalization step, because i had some doubts about the sequencing performance in this kind of samples, since i have only peak after 400-500 bp.
I checked the starting amount, I quantified (Qubit) and I didn't load more than 1ng.
The tagmentation step was like that in the manual (55ºC > 5min).
Any idea from what could influenced the tagmentation?
If it was you, did you continued to sequence?
Thanks in advance
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