Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • easyRNAseq problem: Error in aggregate.data.frame

    easyRNAseq terminates with the error:

    Error in aggregate.data.frame(as.data.frame(x), ...) :
    no rows to aggregate


    I use easyRNAseq to enter my data for DESeq:

    > library(easyRNASeq)
    > library(BSgenome.Hsapiens.UCSC.hg19)
    > setwd('/home/sylvian/SerumFedStarved/DESeq/')
    > chr.sizes=seqlengths(Hsapiens)
    > bamfiles=dir(getwd(), pattern='*.bam$')
    > conditions=c('fed','starved','fed','starved','fed','starved')
    > names(conditions)<-c('DRSNpilot1_STARhg19_paired_sorted.bam','DRSNpilot2_STARhg19_paired_sorted.bam','TQ24_STARhg19_paired_sorted.bam','TQ25_STARhg19_paired_sorted.bam','TQ28_STARhg19_paired_sorted.bam','TQ29_STARhg19_paired_sorted.bam')
    > rnaseqgenes<-easyRNASeq(filesDirectory=getwd(),
    + organism='Hsapiens',
    + chr.sizes=chr.sizes,
    + readLength=100L,
    + annotationMethod='biomaRt',
    + format='bam',
    + count='genes',
    + summarization='geneModels',
    + filenames=bamfiles,
    + conditions=conditions,
    + normalization=TRUE,
    + fitType='local',
    + outputFormat='DESeq')
    Checking arguments...
    Fetching annotations...
    Computing gene models...
    Summarizing counts...
    Processing DRSNpilot1_STARhg19_paired_sorted.bam
    Error in aggregate.data.frame(as.data.frame(x), ...) :
    no rows to aggregate

    Has anybody by any chance had the same problem and knows how to fix this?

    I used to do
    chr.sizes=as.list(seqlengths(Hsapiens)
    but this now produces an error:
    Error in easyRNASeq(filesDirectory = getwd(), organism = "Hsapiens", chr.sizes = chr.sizes, :
    chr.sizes should be a named list of integers. 'Use 'as.integer' to convert from numeric.


    chr.sizes=seqlengths(Hsapiens) produces

    chr1 chr2 chr3
    249250621 243199373 198022430
    chr4 chr5 chr6
    191154276 180915260 171115067
    chr7 chr8 chr9
    159138663 146364022 141213431
    ...

    Thanks a lot

Latest Articles

Collapse

  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
31 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
32 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
28 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
53 views
0 likes
Last Post seqadmin  
Working...
X