Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Split long fasta seq into smaller seqs by removing 'n'

    Hello,

    My fasta file has a long consensus sequence (gigabases long) that is padded with 'n' between the actual sequences. Like this:

    actgggacnnnnnnnnnnnnnnnnnnnnnnnnnnactgacgtggattgc
    aatnnnnnnnnnnnnnnnaccaattggatagagaccnnn

    I've searched rather intensively to see if the internet can solve my problem. Not really, though they were close. For example, people report splitting the long sequence into smaller files but I do not want that. I want the n removed and have all sequences in the same file. Better yet, with the start and end of the sequences.

    Desired output (>seqname_start_end) :
    >seq1_1_8
    actgggac

    >seq2_35_52
    actgacgtggattgcaat

    >seq3_68_85
    accaattggatagagacc

    If anyone could point me towards a right tool (bioperl, etc) or give me a pseudo-code in perl, I would appreciate it.

    Thanks.

  • #2
    IDBA contains a script called split_scaffold

    Contribute to loneknightpy/idba development by creating an account on GitHub.


    Here's what it does:

    IN =>

    >test
    acgacgacgacgacgacgacgacagcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnacgacgacgacagcagtagatgatgatagtag


    OUT =>

    >teste_0
    ACGACGACGACGACGACGACGACAGCA
    >teste_1
    ACGACGACGACAGCAGTAGATGATGATAGTAG

    Comment


    • #3
      Originally posted by azneto View Post
      IDBA contains a script called split_scaffold

      Contribute to loneknightpy/idba development by creating an account on GitHub.

      Great! Thanks a lot! Worked flawlessly.

      It doesn't get me the start/end of the base positions but that I can live with.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
        04-22-2024, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Today, 08:47 AM
      0 responses
      10 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      60 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      57 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      53 views
      0 likes
      Last Post seqadmin  
      Working...
      X