Hi,
I got several rpkm values for one gene (gene.expr file, not tranascript) when I use cufflinks. I trimmed my reads because of bad quality score. I run Tophat for different lengthes separately. Then I combined all the different length of result (accepted.sam file) and sorted them according to the start site for each chromosome, after that I run cufflinks, I found I got several rpkm values for one gene. Does anyone can explain this and how to solve it?
I got several rpkm values for one gene (gene.expr file, not tranascript) when I use cufflinks. I trimmed my reads because of bad quality score. I run Tophat for different lengthes separately. Then I combined all the different length of result (accepted.sam file) and sorted them according to the start site for each chromosome, after that I run cufflinks, I found I got several rpkm values for one gene. Does anyone can explain this and how to solve it?
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