Hello,
I'm trying to perform rRNA depletion on my total extracted RNA from a panel of E. coli isolates using the Ribo-Zero bacteria kit but am getting extremely low recoverable yields (around 1-2 ng total RNA). I've been informed I need 10 ng of RNA in 5 µl total volume for library prep for RNA-seq. I've run the before and after samples on the bioanalyser and it seems fine before performing rRNA depletion. I've tried all the Illumina recommended post depletion purification methods (ETOH ppt, Qiagen mini-elute and clean up and zymo clean and concentrator columns) but sadly this seems to be as good as it gets.
Any advice would be greatly appreciated!
Thanks
I'm trying to perform rRNA depletion on my total extracted RNA from a panel of E. coli isolates using the Ribo-Zero bacteria kit but am getting extremely low recoverable yields (around 1-2 ng total RNA). I've been informed I need 10 ng of RNA in 5 µl total volume for library prep for RNA-seq. I've run the before and after samples on the bioanalyser and it seems fine before performing rRNA depletion. I've tried all the Illumina recommended post depletion purification methods (ETOH ppt, Qiagen mini-elute and clean up and zymo clean and concentrator columns) but sadly this seems to be as good as it gets.
Any advice would be greatly appreciated!
Thanks
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