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  • SNP calling from a reference sequence

    Hello, I am looking for SNPs in the rDNA of fungal genomes.
    I have a consensus sequence (~100 bps), and a lot of reads taken from a WGS (~900 bps) that align to it, the reads are provided together with their quality scores.
    I'd like to look for SNPs among these reads, is there a software ready to use or should I write one by myself? I have seen MAQ, but it seems it works only with short reads. Do you have any suggestions?

    thank you

  • #2
    I forgot to say that I am mostly interested in variations than indels

    Originally posted by blackrabite View Post
    Hello, I am looking for SNPs in the rDNA of fungal genomes.
    I have a consensus sequence (~100 bps), and a lot of reads taken from a WGS (~900 bps) that align to it, the reads are provided together with their quality scores.
    I'd like to look for SNPs among these reads, is there a software ready to use or should I write one by myself? I have seen MAQ, but it seems it works only with short reads. Do you have any suggestions?

    thank you

    Comment


    • #3
      GSNAP/GMAP works very well for us. Here is the link.

      Comment

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