I have run a batch of small RNA samples on the SOLiD system. Following sequencing, I've trimmed the adapters (in colour space) using CLC Bio.
The statistics are as follows...
Reads Trimmed: 98.1%
Average Length after trim: 33.7bp
It is the latter figure that is worrying me...just wondering if anyone has experienced this? My expectation was a read average of ~22 and only 15,000reads fit this catergory.
Cheers
D
The statistics are as follows...
Reads Trimmed: 98.1%
Average Length after trim: 33.7bp
It is the latter figure that is worrying me...just wondering if anyone has experienced this? My expectation was a read average of ~22 and only 15,000reads fit this catergory.
Cheers
D
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