Hi all,
I am a rookie in terms of NGS and am currently pursuing a project in Puerto Montt, Chile on microbial diversity and community composition. Due to lack of resources, Macrogen (in Korea) is performing the majority of the bioinformatics-preparation of amplicon libraries and sequencing. With a total of 18 samples, I was recently and unfortunately informed that 5 samples failed, with "no peak" in the Agilent bioanalyzer & Caliper Labchip GX DNA High Sensitivity portion of the project. I have attached the failed and successful results (three attachments) and am curious if anyone has any advice as to 1. what went wrong and 2. what could be the next step with these failed samples. Any help/advice would be appreciated, thanks.
Katie
I am a rookie in terms of NGS and am currently pursuing a project in Puerto Montt, Chile on microbial diversity and community composition. Due to lack of resources, Macrogen (in Korea) is performing the majority of the bioinformatics-preparation of amplicon libraries and sequencing. With a total of 18 samples, I was recently and unfortunately informed that 5 samples failed, with "no peak" in the Agilent bioanalyzer & Caliper Labchip GX DNA High Sensitivity portion of the project. I have attached the failed and successful results (three attachments) and am curious if anyone has any advice as to 1. what went wrong and 2. what could be the next step with these failed samples. Any help/advice would be appreciated, thanks.
Katie
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