Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Nextera XT sample prep from genomic samples - question about QC along the way mdalessio Illumina/Solexa 18 10-22-2013 06:07 AM
How to prep BACs for Nextera XT DNA kit? OnUrMark Sample Prep / Library Generation 3 05-20-2013 11:56 AM
Did you know you can save ~90% on your Nextera Sample Prep cost by miniaturization? SAV Introductions 5 03-14-2013 02:25 AM
input DNA for Illumina´s Nextera XT sample prep kit alpinedna Sample Prep / Library Generation 1 11-05-2012 06:29 AM
Sample/library prep of DNA and RNA in a metagenomic sample chrisaw01 Metagenomics 1 05-05-2011 01:59 PM

Thread Tools
Old 01-15-2014, 08:40 AM   #1
Location: France

Join Date: Jul 2013
Posts: 20
Default Miltenyi Microbeads + Nextera DNA Sample Prep

Hi All,

I've been having some trouble purifying my cells by FACS and have been considering moving to the Miltenyi microbead sorting system. I'm trying to figure out whether the magnetic beads (which are small) will interfere with the cDNA synthesis and/or library preparation step. I figure they will probably be eliminated along with the Agencourt AMPure Library cleanup step but I'm not sure if it will screw anything up prior. Does anyone have any experience with this?

Thanks for your help!
travelk is offline   Reply With Quote

magnetic beads, microbead, miltenyi

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 04:11 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO