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Old 06-20-2018, 01:17 PM   #1
grace_pang
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Default Adaptor length of Ligation Sequencing Kit 1D

Does anyone know the sequence or at lease length of the adaptor from Nanopore Ligation Sequencing Kit 1D?

I'm trying to figure out how much Ampure Beads to use to get rid of short fragments, so the length will be helpful.


Thank you!
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Old 06-20-2018, 02:11 PM   #2
wdecoster
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If you have access to the ONT community website you can find the adapter sequences here: https://community.nanoporetech.com/t...ing-kit-family

They're about 60 nucleotides and 20 nucleotides (y-shaped). I probably cannot disclose the exact sequences.here.

Last edited by wdecoster; 06-20-2018 at 02:28 PM.
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Old 06-21-2018, 04:50 AM   #3
grace_pang
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Thank you wdecoster! Right now I couldn't login to the community because ONT messed up my login.. They are trying to figure out what went wrong..
But the length is all what I want right now. Thank you!
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