I prepare genomic shotgun libraries for sequencing using Single Molecule Real Time (SMRT) sequencing platform produced by PacBio (20X coverage of PacBio reads) coupled with sequencing on Illumina platform (30X coverage of Illumina Miseq 250bp reads).The obtained data will be used to perform a de novo hybrid assembly by combining both Illumina and PacBio reads to generate accurate draft assemblies of plant genome (with estimated size of 350 Mbp).
1- What types of assembler I should use?
2-A computer equipped with 320 RAM, 3TB hard disc and 2 x Xeon 2680v2 is it sufficient to analyze the results of sequencing and to make a hybrid assembly?
1- What types of assembler I should use?
2-A computer equipped with 320 RAM, 3TB hard disc and 2 x Xeon 2680v2 is it sufficient to analyze the results of sequencing and to make a hybrid assembly?
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