Go Back   SEQanswers > Sequencing Technologies/Companies > 454 Pyrosequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
Difference between 454 pair end library and 454 standard library edge 454 Pyrosequencing 1 09-30-2009 02:08 AM

Thread Tools
Old 12-11-2008, 02:09 AM   #1
Junior Member
Location: Germany

Join Date: Aug 2008
Posts: 2
Default idle 454 ?

Hello 454 users,

I have few questions regarding saving power and operating 454 in an environment-friendly way

1. Is it wise to shutdown the idle 454 machines?
2. Does shutting down (in idle time) often, result in inconsistencies of sequencing runs?
3. Does it affect the lifespan and performance of the CCD Camera and laser beamer?

These questions also holds good for solexa machines as well.

Thank you in advance for your time and answers,

geevom is offline   Reply With Quote
Old 11-09-2010, 09:39 AM   #2
Location: Prague, Czech Republic

Join Date: Nov 2010
Posts: 40

I had a lot of similar questions around, it seems that the camera housing has some fitting which "might" be optimized for cooled temperature. The camera itself is an "ordinary" astronomical camera. ;-) When a machine is left in a package in room temperature (like a year or so) the vacuum will drop. However, Roche engineer can come and re-new the vacuum in there. Another reason I was told is that the 4 peristaltic pumps move all the time a bit back and forth. When turned off, maybe they will have a bit problem to move smoothly after startup, or the tubing will harden meanwhile and crack. The camera should not be creating much heat and the cooling is advantageous to minimize the noise while capturing data.

Personally, I doubt it matters.

Last edited by martin2; 11-09-2010 at 10:15 AM. Reason: shortened
martin2 is offline   Reply With Quote


Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 08:18 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO