Hi!
I am planning to perform MeDip-seq experiments. Based on my samples, I plan to recover very little amount of methylated DNA. Therefore, I was thinking to perform an amplification step on the immunoprecipitated material before adapter ligation and gel size selection. Has anybody experience in some method of amplification that minimizes the introduction of bias in the experiment?
Thank you very much!
MGiulia
I am planning to perform MeDip-seq experiments. Based on my samples, I plan to recover very little amount of methylated DNA. Therefore, I was thinking to perform an amplification step on the immunoprecipitated material before adapter ligation and gel size selection. Has anybody experience in some method of amplification that minimizes the introduction of bias in the experiment?
Thank you very much!
MGiulia
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