Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • finding Transposable Elements using RepeatMasker

    down vote
    favorite
    I have been trying to detect, classify the transposable element in my eukaryotic non-referenced genome, (denovo)

    I'm using RepeatMasker to do the job but whenever I run the tools the results shows only simple repeat and satellites.

    where I'm trying to detect LTR elements, LINEs, SINES

    bases masked: 33282890 bp ( 1.66 %)
    ==================================================
    number of length percentage
    elements* occupied of sequence
    --------------------------------------------------
    SINEs: 0 0 bp 0.00 %
    Alu/B1 0 0 bp 0.00 %
    MIRs 0 0 bp 0.00 %

    LINEs: 0 0 bp 0.00 %
    LINE1 0 0 bp 0.00 %
    LINE2 0 0 bp 0.00 %
    L3/CR1 0 0 bp 0.00 %
    RTE 0 0 bp 0.00 %

    LTR elements: 0 0 bp 0.00 %
    ERVL 0 0 bp 0.00 %
    ERVL-MaLRs 0 0 bp 0.00 %
    ERV_classI 0 0 bp 0.00 %
    ERV_classII 0 0 bp 0.00 %

    DNA elements: 0 0 bp 0.00 %
    hAT-Charlie 0 0 bp 0.00 %
    TcMar-Tigger 0 0 bp 0.00 %

    Unclassified: 0 0 bp 0.00 %

    Total interspersed repeats: 0 bp 0.00 %


    Small RNA: 0 0 bp 0.00 %

    Satellites: 1 267 bp 0.00 %
    Simple repeats: 663679 27953718 bp 1.39 %
    Low complexity: 123705 6154714 bp 0.31 %



    any idea or suggestions can help me out, please tq

  • #2
    Hi! RepeatMasker identifies interspersed repeats by comparing it with predefined sequence libraries. Your species might just have different repeats from the tables that RepearMasker uses. At the same website you will find RepeatModeler, which might fit your needs better, I think...

    Comment


    • #3
      thanks for the response

      what I understand from your replay that I have to create a special library from target genome with Repeatmodeler then run Repeatmasker again?

      is there any suggestions for a suitable tool that can scan this element out of the sequence

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin


        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
        Yesterday, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      55 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      52 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      45 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      55 views
      0 likes
      Last Post seqadmin  
      Working...
      X