Hi All,
I am part of a sequencing core, trying to QC a client's ATAC-Seq libraries via the bioanalyzer on a DNA high sensitivity chip. Prior to running the samples I checked the concentrations with a qubit fluorometer and diluted them all to ~4-6 ng/uL. Perhaps a little high for the high sensitivity chip, but not enough to overload it.
After talking with the client I believed the problems was long fragments carrying over between samples, so I tried to space them out, only loading samples in position 1, 4, 7, and 10. Unfortunately, I am still getting garbage traces after the first sample. Does anyone have any recommendations for how to handle these samples?
I've attached the traces as an example - you can see the characteristic ATAC peaks in sample 1, and then some higher molecular weight material near the marker, continuing into the next two traces.
I am part of a sequencing core, trying to QC a client's ATAC-Seq libraries via the bioanalyzer on a DNA high sensitivity chip. Prior to running the samples I checked the concentrations with a qubit fluorometer and diluted them all to ~4-6 ng/uL. Perhaps a little high for the high sensitivity chip, but not enough to overload it.
After talking with the client I believed the problems was long fragments carrying over between samples, so I tried to space them out, only loading samples in position 1, 4, 7, and 10. Unfortunately, I am still getting garbage traces after the first sample. Does anyone have any recommendations for how to handle these samples?
I've attached the traces as an example - you can see the characteristic ATAC peaks in sample 1, and then some higher molecular weight material near the marker, continuing into the next two traces.
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