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  • Samtools IO error

    Hi Experts,
    I am trying to use samtools to remove duplicates and bad reads from a BAM file. I get the following IO error. Does this mean I don't have enough space for output generation?

    Exception in thread "main" net.sf.samtools.util.RuntimeIOException: sync failed
    at net.sf.samtools.util.BinaryCodec.close(BinaryCodec.java:594)
    at net.sf.samtools.util.BlockCompressedOutputStream.close(BlockCompressedOutputStream.java:178)
    at java.io.FilterOutputStream.close(FilterOutputStream.java:143)
    at net.sf.samtools.util.BinaryCodec.close(BinaryCodec.java:590)
    at net.sf.samtools.BAMFileWriter.finish(BAMFileWriter.java:77)
    at net.sf.samtools.SAMFileWriterImpl.close(SAMFileWriterImpl.java:175)
    at FilterBAMForSNPCalling.main(FilterBAMForSNPCalling.java:57)
    Caused by: java.io.SyncFailedException: sync failed
    at java.io.FileDescriptor.sync(Native Method)
    at net.sf.samtools.util.BinaryCodec.close(BinaryCodec.java:588)

    Thanks for your input.
    Uma

  • #2
    UPDATE:
    This error occured because I didn't specify the directory as current in the msub command:
    echo "command"| msub -V -d `pwd`...
    Moab was trying to write the output in a directory where I did not have permissions.

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