Hi,
BLAST 2.2.30+
I've 16 nucleotide base sequences, which I'd like to use to gather pairwise identity against another FASTA file.
This works fine, I get 3 files ending in with .nhr, and likewise.
For some reason(s) I don't get desired results using this DB.
I go ahead and BLAST my input file against itself.
I get no hits everywhere.
with -outfmt flag, and option 6 I get empty file.
Tried with ' and " with -outfmt 6 to get desired results, but in vain.
I used -dust flag with option no I see no change in output
I'm running out of options to try to have desired results.
Any help will be greatly appreciated.
I've looked for help at:
BLAST 2.2.30+
I've 16 nucleotide base sequences, which I'd like to use to gather pairwise identity against another FASTA file.
Code:
makeblastdb -in barcode.fasta -dbtype nucl -out barcode.fasta -title newdb
For some reason(s) I don't get desired results using this DB.
I go ahead and BLAST my input file against itself.
Code:
blastn -query barcode.fasta -out ./3blasted_seq.txt -subject barcode.fasta
with -outfmt flag, and option 6 I get empty file.
Tried with ' and " with -outfmt 6 to get desired results, but in vain.
I used -dust flag with option no I see no change in output
I'm running out of options to try to have desired results.
Any help will be greatly appreciated.
I've looked for help at:
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