Hi there! I have a silly question about the MiSeq read length.
I'm going to run a MiSeq plate of 16s amplicons.
When they talk about 2x250bp reads, do they include the adaptor in the calculation? So, can I expect "useful" 250bp or do I need to subtract the adaptor length?
Thanks
Francesca
I'm going to run a MiSeq plate of 16s amplicons.
When they talk about 2x250bp reads, do they include the adaptor in the calculation? So, can I expect "useful" 250bp or do I need to subtract the adaptor length?
Thanks
Francesca
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